We, therefore, recommend adding human serum or albumin solution. Autogenic serum is not required. Suitable accessories The available quantity and the cell content of the samples determine the adequate chamber. Slide preparations of liquor cerebrospinalis usually require dry fixation.
For this reason, the insert should be assembled witha filter card see illustration B1 in above mentioned leaflet. When working with infectious samples, the inserts should be closed with hygienically sealed lid no. Centrifugation a Sedimentation Centrifuge the cyto chambers for 3 minutes at x g this corresponds to 1, min-1 with the 6-place rotor and 1, min-1 with the 4-place rotor. During centrifugation, the cells sediment onto the slide.
[Haematology] Cerebrospinal Fluid Analysis - Laboratory Examination
When draining the chamber, take care not to stir the sediment. Otherwise, the quality of the preparation will suffer and cells may get lost.
We advise to use a Pasteur pipette for removing the supernatant. Do not position the tip of the pipette on the slide, but follow the liquid level with it carefully downwards. Do not drain the chamber completely, but leave a drop of supernatant on the sediment! If no albumin has been added to the sample, the recovered supernatant may be used for biochemical analyses.
Dry the sediment by centrifuging it a second time. After the supernatant has been removed, twist off the fastening ring and lift it off with the chamber see illustration B4. Put the cyto suspension with slide carrier, slide and filter card back into the centrifuge and spin for 1 minute at 1, x g this corresponds to 3, min-1 with the 6-place rotor and 3, min-1with the 4-place rotor.
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The residual supernatant is spun off by centrifugal force and absorbed by the filter card. The cells which constitute the sediment stay on the slide.
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They are well preserved and ideally spread. By drying the preparation by centrifugation, evaporation artefacts like shrunk leucocytes or crystallisations can be avoided.
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Put the slides rinsed with Weise buffer into the frames, and put the frames into the centrifuge. Review as guest.
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Publication Date:. Look Inside. Table of Contents. Product Description. Highlights: Guidelines for the proper handling of specimens, cell preparation, and staining techniques Review of the common sources of error in diagnosis Thorough coverage of the techniques for detecting and classifying inflammatory, infectious, neoplastic, and hemorrhagic conditions of the central nervous system Descriptions of the principle features of cells and the classification of tumor cell types according to current W.
Color Atlas of Body Fluids: An Illustrated Field Guide Based on Proficiency Testing-PUB
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